Seamless cloning and gene fusion
نویسندگان
چکیده
منابع مشابه
Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
Background: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. Methods: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested ...
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Synthesizing two images with seamless boundaries, i.e. seamless image cloning, is important and has many useful applications in CG. Previous approaches do not produce realistic results if texture details of the two images are different. We propose a novel texture-aware seamless cloning framework based on separately processing the details and base image colors. The proposed framework provides re...
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Here, an efficient cloning strategy for large DNA fragments and for simultaneous assembly of multiple DNA fragments assembly is presented. This strategy is named OEPR (based on Overlap Extension PCR and Recombination in vivo). OEPR cloning is a seamless, restriction- and ligation-independent method. The method takes advantage of both homologous recombination enzymes in E. coli and overlap PCR. ...
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ژورنال
عنوان ژورنال: Trends in Biotechnology
سال: 2005
ISSN: 0167-7799
DOI: 10.1016/j.tibtech.2005.02.008